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resource source identifier puc19 linearized vector takara bio 638947 puc19 pssp17  (TaKaRa)


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    TaKaRa resource source identifier puc19 linearized vector takara bio 638947 puc19 pssp17
    Resource Source Identifier Puc19 Linearized Vector Takara Bio 638947 Puc19 Pssp17, supplied by TaKaRa, used in various techniques. Bioz Stars score: 98/100, based on 1112 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/source+vector/In-Fusion+Snap+Assembly+Master+Mix/pm41321633-115-2-8
    Average 98 stars, based on 1112 article reviews
    resource source identifier puc19 linearized vector takara bio 638947 puc19 pssp17 - by Bioz Stars, 2026-11
    98/100 stars

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    Related Articles

    other:

    Article Title: SMARCA4 is a haploinsufficient B cell lymphoma tumor suppressor that fine-tunes centrocyte cell fate decisions.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Raw single ATAC sequencing FASTQ files This paper GSE254908 Raw ATAC-seq FASTQ files This paper GSE254908 Raw RNA-sequencing FASTQ files This paper GSE254908 Gene expression microarray data for primary BL GEO GSE132929 Experimental models: Cell lines Human: Raji cells ATCC CCL-86 Human: HEK293T cells ATCC RRID:CVCL_0063 Experimental models: Organisms/strains Mouse: C57BL/6J The Jackson Laboratory Stock# 000664 Mouse: Cd19cre (B6.129P2(C)- Cd19tm1(cre)Cgn/J) The Jackson Laboratory Stock# 006785 Mouse: AID Cre (B6.129P2-Aicdatm1(cre)Mnz/J) The Jackson Laboratory Stock# 007770 Mouse: CD45.1 (B6.SJL-PtprcaPepcb/Boy) The Jackson Laboratory Stock# 002014 Mouse: Rag1 KO (B6.129S7-Rag1tm1Mom/J) The Jackson Laboratory Stock# 002216 Mouse: R26StopFLMYC (C57BL/6N-Gt (ROSA)26Sortm13(CAG-MYC,-CD2*)Rsky/J) The Jackson Laboratory Stock# 020458 Mouse: GFP-Myc:B6;129-Myctm1Slek/J The Jackson Laboratory Stock#021935 Oligonucleotides gRNA1 for the N-terminal SMARCA4 tagging: TCTGGAGTGGACATCTTCAC This manuscript N/A gRNA2 for the N-terminal SMARCA4 tagging: GTCTGGAGTGGACATCTTCA This manuscript N/A AID-GFP-SMARCA4 N-terminal genotyping Fwd: 5’-CGTCTCCCAAGTTCAAGCGATT-3’ This manuscript N/A AID-GFP-SMARCA4 N-terminal genotyping Rev: 5’-ACATCCAGGGACATGCAAAAGA-3’ This manuscript N/A gRNA1 for knock-in of SMARCA4 point mutation: CAGGACCGAGCCCACCGCAT This manuscript N/A gRNA2 for knock-in of SMARCA4 point mutation: CCGCATCGGGCAGCAGAACG This manuscript N/A Smarca4 mouse genotyping Fwd: 5’—3’ This manuscript N/A Smarca4 mouse genotyping Rev: 5’—3’ This manuscript N/A AID-Cre mouse genotyping common Fwd: 5’- GGACCCAACCCAGGAGGCAGATGT-3’ Robbiani et al.66 N/A AID-Cre mouse genotyping mutant Rev: 5’- CACTCGTTGCATCGACCGGTAATG-3’ Robbiani et al.66 N/A AID-Cre mouse genotyping wildtype Rev: 5’- CCTCTAAGGCTTCGCTGTTATTACCAC-3’ Robbiani et al.66 N/A Myc-KI mouse wildtype Fwd: 5’-CCAAAGTCGCTCTGAGTTGTTATC-3’ Calado et al.25 N/A Myc-KI mouse wildtype Rev: 5’-GAGCGGGAGAAATGGATATG-3’ Calado et al.25 N/A Myc-KI mouse mutant Fwd: 5’-CCAAGAGGGTCAAGTTGGA-3’ Calado et al.25 N/A Myc-KI mouse mutant Rev: 5’-GCAATATGGTGGAAAATAAC-3’ Calado et al.25 N/A GFP-myc mouse common Fwd: 5’- CTGAAAGGGGAGTGGTTCAG-3’ Huang et al.28 N/A GFP-myc mouse wildtype Rev: 5’- ACCGCAACATAGGATGGAGA-3’ Huang et al.28 N/A GFP-myc mouse mutant Rev: 5’- GAACTTCAGGGTCAGCTTGC-3’ Huang et al.28 N/A (Continued on next page) e4 Cancer Cell 42, 605–622.e1–e11, April 8, 2024

    Recombinant:

    Article Title: Nuclear transport surveillance of p53 by nuclear pores in glioblastoma.
    Article Snippet: Experimental models: Organisms/strains BALB/cSlc-nu/nu mice Charles River Laboratories, Osaka, Japan N/A Oligonucleotides shRNA oligo sequence, see Table S1 This paper N/A Primers for quantitative real-time PCR, see Table S2 This paper N/A (Continued on next page) Cell Reports 42, 112882, August 29, 2023 13 .. REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA pDsRed-Monomer-C1 vector Clontech Cat# Z2466N HindIII New England BioLabs Cat# R0104 BamHI TOYOBO Cat# BAH-111 DsRed-p53DNES vector This paper N/A EcoRI BioLabs Cat# R0101 Software and algorithms LAS X version 1.8 Leica N/A FV10-ASW4.2 Olympus N/A Graph Pad Prism 7 Graph Pad N/A Multi Gauge V3.2 FUJIFILM Wako Pure Chemicals N/A Other flow view FV10i Olympus N/A LAS-4000 image analyzer FUJIFILM Wako Pure Chemicals N/A Multiskan GO Thermo Fisher Scientific N/A PCR Thermal Cycler Dice Takara N/A Thermal Cycler Dice Real time Takara N/A TCS SP8 Leica N/A ..

    Article Title: The Arabidopsis blue-light photoreceptor CRY2 is active in darkness to inhibit root growth.
    Article Snippet: Article The Arabidopsis blue-light photoreceptor CRY2 is active in darkness to inhibit root growth

    Software:

    Article Title: Nuclear transport surveillance of p53 by nuclear pores in glioblastoma.
    Article Snippet: Experimental models: Organisms/strains BALB/cSlc-nu/nu mice Charles River Laboratories, Osaka, Japan N/A Oligonucleotides shRNA oligo sequence, see Table S1 This paper N/A Primers for quantitative real-time PCR, see Table S2 This paper N/A (Continued on next page) Cell Reports 42, 112882, August 29, 2023 13 .. REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA pDsRed-Monomer-C1 vector Clontech Cat# Z2466N HindIII New England BioLabs Cat# R0104 BamHI TOYOBO Cat# BAH-111 DsRed-p53DNES vector This paper N/A EcoRI BioLabs Cat# R0101 Software and algorithms LAS X version 1.8 Leica N/A FV10-ASW4.2 Olympus N/A Graph Pad Prism 7 Graph Pad N/A Multi Gauge V3.2 FUJIFILM Wako Pure Chemicals N/A Other flow view FV10i Olympus N/A LAS-4000 image analyzer FUJIFILM Wako Pure Chemicals N/A Multiskan GO Thermo Fisher Scientific N/A PCR Thermal Cycler Dice Takara N/A Thermal Cycler Dice Real time Takara N/A TCS SP8 Leica N/A ..

    Article Title: Evaluation of Plasmodium falciparum PSSP17 essentiality in human-to-mosquito transmission and its prospective candidacy as a biomarker.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER pUC19 linearized vector Takara Bio 638947 pUC19-PSSP17 This study N/A Software and algorithms SnapGene https://www.snapgene.com 8.1.1 EuPaGDT http://grna.ctegd.uga.edu/ N/A PathWeaver (default parameters) https://github.com/nickjhathaway/ PathWeaver N/A Alphafold (default parameters) Varadi et al. 46 N/A UCSF ChimeraX Pettersen et al. 47 N/A iScience 28, 113919, December 19, 2025 e2 pUC19 via In-Fusion cloning. ..

    Polymerase Chain Reaction:

    Article Title: Nuclear transport surveillance of p53 by nuclear pores in glioblastoma.
    Article Snippet: Experimental models: Organisms/strains BALB/cSlc-nu/nu mice Charles River Laboratories, Osaka, Japan N/A Oligonucleotides shRNA oligo sequence, see Table S1 This paper N/A Primers for quantitative real-time PCR, see Table S2 This paper N/A (Continued on next page) Cell Reports 42, 112882, August 29, 2023 13 .. REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant DNA pDsRed-Monomer-C1 vector Clontech Cat# Z2466N HindIII New England BioLabs Cat# R0104 BamHI TOYOBO Cat# BAH-111 DsRed-p53DNES vector This paper N/A EcoRI BioLabs Cat# R0101 Software and algorithms LAS X version 1.8 Leica N/A FV10-ASW4.2 Olympus N/A Graph Pad Prism 7 Graph Pad N/A Multi Gauge V3.2 FUJIFILM Wako Pure Chemicals N/A Other flow view FV10i Olympus N/A LAS-4000 image analyzer FUJIFILM Wako Pure Chemicals N/A Multiskan GO Thermo Fisher Scientific N/A PCR Thermal Cycler Dice Takara N/A Thermal Cycler Dice Real time Takara N/A TCS SP8 Leica N/A ..

    Plasmid Preparation:

    Article Title: Hue-shifted monomeric variants of Clavularia cyan fluorescent protein: identification of the molecular determinants of color and applications in fluorescence imaging
    Article Snippet: .. Thus, to prepare mTFP1 and mWasabi N-terminal fusions, the following digests were performed: human non-muscle α-actinin, EcoRI and NotI (vector source, Tom Keller, FSU); human cytochrome C oxidase subunit VIII, BamHI and NotI (mitochondria, Clontech); human zyxin, BamHI and NotI (Clare Waterman-Storer, NIH); rat α-1 connexin-43 and rat β-2 connexin-26, EcoRI and BamHI (Matthias Falk, Lehigh University); human H2B, BamHI and NotI (George Patterson, NIH); N-terminal 81 amino acids of human β-1,4-galactosyltransferase, BamHI and NotI (Golgi, Clontech); human microtubule-associated protein EB3, BamHI and NotI (Lynne Cassimeris, Lehigh University); human vimentin, BamHI and NotI (Robert Goldman, Northwestern University); human keratin 18, EcoRI and NotI (Open Biosystems, Huntsville, AL); chicken paxillin, EcoRI and NotI (Alan Horwitz, University of Virginia); rat lysosomal membrane glycoprotein 1, AgeI and NheI (George Patterson, NIH); endoplasmic reticulum (calreticulin signal sequence and KDEL retention sequence), AgeI and EcoRI (Clontech). .. To prepare mTFP1 and mWasabi C-terminal fusions, the following digests were performed: human β-actin, NheI and BglII (Clontech); human α-tubulin, NheI and BglII (Clontech); human light chain clathrin, NheI and BglII (George Patterson, NIH); human lamin B1, NheI and BglII (George Patterson, NIH); human fibrillarin, AgeI and BglII (Evrogen); human vinculin, NheI and EcoRI (Open Biosystems, Huntsville, AL); peroximal targeting signal 1 (PTS1 – peroxisomes), AgeI and BspEI (Clontech); chicken protein tyrosine kinase 2, AgeI and BglII (Clare Waterman-Storer, NIH); human annexin (A4), AgeI and BspEI (Alen Piljic, EMBL, Heidelberg); human RhoB GTPase with an N-terminal c-Myc epitope tag (endosomes), AgeI and BspEI (Clontech); and the 20-amino acid farnesylation signal from c-Ha-Ras, AgeI and BspEI (membrane, Clontech).

    Article Title: A New Strategy for Nucleic Acid Delivery and Protein Expression Using Biocompatible Nanohydrogels of Predefined Sizes
    Article Snippet: .. The unmodified vector can be used to express mCherry in mammalian cells (source: www.takarabio.com ; catalog number: 632523). .. The plasmid DNA was amplified in E. coli and purified according to the supplier’s protocol (Promega, Madison, WI, USA).

    Article Title: The Arabidopsis blue-light photoreceptor CRY2 is active in darkness to inhibit root growth.
    Article Snippet: Article The Arabidopsis blue-light photoreceptor CRY2 is active in darkness to inhibit root growth

    Article Title: Evaluation of Plasmodium falciparum PSSP17 essentiality in human-to-mosquito transmission and its prospective candidacy as a biomarker.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER pUC19 linearized vector Takara Bio 638947 pUC19-PSSP17 This study N/A Software and algorithms SnapGene https://www.snapgene.com 8.1.1 EuPaGDT http://grna.ctegd.uga.edu/ N/A PathWeaver (default parameters) https://github.com/nickjhathaway/ PathWeaver N/A Alphafold (default parameters) Varadi et al. 46 N/A UCSF ChimeraX Pettersen et al. 47 N/A iScience 28, 113919, December 19, 2025 e2 pUC19 via In-Fusion cloning. ..

    Article Title: A New Strategy for Nucleic Acid Delivery and Protein Expression Using Biocompatible Nanohydrogels of Predefined Sizes.
    Article Snippet: .. The unmodified vector can be used to express mCherry in mammalian cells (source: www.takarabio.com; catalog number: 632523). .. The plasmid DNA was amplified in E. coli and purified according to the supplier’s protocol (Promega, Madison, WI, USA).

    Membrane:

    Article Title: Hue-shifted monomeric variants of Clavularia cyan fluorescent protein: identification of the molecular determinants of color and applications in fluorescence imaging
    Article Snippet: .. Thus, to prepare mTFP1 and mWasabi N-terminal fusions, the following digests were performed: human non-muscle α-actinin, EcoRI and NotI (vector source, Tom Keller, FSU); human cytochrome C oxidase subunit VIII, BamHI and NotI (mitochondria, Clontech); human zyxin, BamHI and NotI (Clare Waterman-Storer, NIH); rat α-1 connexin-43 and rat β-2 connexin-26, EcoRI and BamHI (Matthias Falk, Lehigh University); human H2B, BamHI and NotI (George Patterson, NIH); N-terminal 81 amino acids of human β-1,4-galactosyltransferase, BamHI and NotI (Golgi, Clontech); human microtubule-associated protein EB3, BamHI and NotI (Lynne Cassimeris, Lehigh University); human vimentin, BamHI and NotI (Robert Goldman, Northwestern University); human keratin 18, EcoRI and NotI (Open Biosystems, Huntsville, AL); chicken paxillin, EcoRI and NotI (Alan Horwitz, University of Virginia); rat lysosomal membrane glycoprotein 1, AgeI and NheI (George Patterson, NIH); endoplasmic reticulum (calreticulin signal sequence and KDEL retention sequence), AgeI and EcoRI (Clontech). .. To prepare mTFP1 and mWasabi C-terminal fusions, the following digests were performed: human β-actin, NheI and BglII (Clontech); human α-tubulin, NheI and BglII (Clontech); human light chain clathrin, NheI and BglII (George Patterson, NIH); human lamin B1, NheI and BglII (George Patterson, NIH); human fibrillarin, AgeI and BglII (Evrogen); human vinculin, NheI and EcoRI (Open Biosystems, Huntsville, AL); peroximal targeting signal 1 (PTS1 – peroxisomes), AgeI and BspEI (Clontech); chicken protein tyrosine kinase 2, AgeI and BglII (Clare Waterman-Storer, NIH); human annexin (A4), AgeI and BspEI (Alen Piljic, EMBL, Heidelberg); human RhoB GTPase with an N-terminal c-Myc epitope tag (endosomes), AgeI and BspEI (Clontech); and the 20-amino acid farnesylation signal from c-Ha-Ras, AgeI and BspEI (membrane, Clontech).

    Sequencing:

    Article Title: Hue-shifted monomeric variants of Clavularia cyan fluorescent protein: identification of the molecular determinants of color and applications in fluorescence imaging
    Article Snippet: .. Thus, to prepare mTFP1 and mWasabi N-terminal fusions, the following digests were performed: human non-muscle α-actinin, EcoRI and NotI (vector source, Tom Keller, FSU); human cytochrome C oxidase subunit VIII, BamHI and NotI (mitochondria, Clontech); human zyxin, BamHI and NotI (Clare Waterman-Storer, NIH); rat α-1 connexin-43 and rat β-2 connexin-26, EcoRI and BamHI (Matthias Falk, Lehigh University); human H2B, BamHI and NotI (George Patterson, NIH); N-terminal 81 amino acids of human β-1,4-galactosyltransferase, BamHI and NotI (Golgi, Clontech); human microtubule-associated protein EB3, BamHI and NotI (Lynne Cassimeris, Lehigh University); human vimentin, BamHI and NotI (Robert Goldman, Northwestern University); human keratin 18, EcoRI and NotI (Open Biosystems, Huntsville, AL); chicken paxillin, EcoRI and NotI (Alan Horwitz, University of Virginia); rat lysosomal membrane glycoprotein 1, AgeI and NheI (George Patterson, NIH); endoplasmic reticulum (calreticulin signal sequence and KDEL retention sequence), AgeI and EcoRI (Clontech). .. To prepare mTFP1 and mWasabi C-terminal fusions, the following digests were performed: human β-actin, NheI and BglII (Clontech); human α-tubulin, NheI and BglII (Clontech); human light chain clathrin, NheI and BglII (George Patterson, NIH); human lamin B1, NheI and BglII (George Patterson, NIH); human fibrillarin, AgeI and BglII (Evrogen); human vinculin, NheI and EcoRI (Open Biosystems, Huntsville, AL); peroximal targeting signal 1 (PTS1 – peroxisomes), AgeI and BspEI (Clontech); chicken protein tyrosine kinase 2, AgeI and BglII (Clare Waterman-Storer, NIH); human annexin (A4), AgeI and BspEI (Alen Piljic, EMBL, Heidelberg); human RhoB GTPase with an N-terminal c-Myc epitope tag (endosomes), AgeI and BspEI (Clontech); and the 20-amino acid farnesylation signal from c-Ha-Ras, AgeI and BspEI (membrane, Clontech).

    Cloning:

    Article Title: Evaluation of Plasmodium falciparum PSSP17 essentiality in human-to-mosquito transmission and its prospective candidacy as a biomarker.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER pUC19 linearized vector Takara Bio 638947 pUC19-PSSP17 This study N/A Software and algorithms SnapGene https://www.snapgene.com 8.1.1 EuPaGDT http://grna.ctegd.uga.edu/ N/A PathWeaver (default parameters) https://github.com/nickjhathaway/ PathWeaver N/A Alphafold (default parameters) Varadi et al. 46 N/A UCSF ChimeraX Pettersen et al. 47 N/A iScience 28, 113919, December 19, 2025 e2 pUC19 via In-Fusion cloning. ..



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